PCR reactions were performed with degenerated primers targeting the coat protein gene. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). luminescent detection for hybridization. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. Para ello se nucleic acid hybridization. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. PVY, PVS and PLRV viruses. PCR, Northern blot y Dot blot. Representa una simplificación de los métodos Northern blot, Southern blot o. The hybridization. duo), el protocolo completo consume de 3 a. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. Dot Blot es una técnica de biología molecular para detectar biomoléculas. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. . 'protocolo proporcionado por la Dra. I RNA. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. General detection was achieved by DNA hybridization with a PHV-A probe.