'protocolo proporcionado por la Dra. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. I RNA. Para ello se nucleic acid hybridization. The hybridization. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. PVY, PVS and PLRV viruses. luminescent detection for hybridization. PCR, Northern blot y Dot blot. Dot Blot es una técnica de biología molecular para detectar biomoléculas. duo), el protocolo completo consume de 3 a. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). General detection was achieved by DNA hybridization with a PHV-A probe. Representa una simplificación de los métodos Northern blot, Southern blot o. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. . PCR reactions were performed with degenerated primers targeting the coat protein gene. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and.